High dissolved oxygen tension triggers outer membrane vesicle formation by Neisseria meningitidis
نویسندگان
چکیده
منابع مشابه
Cysteine Depletion Causes Oxidative Stress and Triggers Outer Membrane Vesicle Release by Neisseria meningitidis; Implications for Vaccine Development
Outer membrane vesicles (OMV) contain immunogenic proteins and contribute to in vivo survival and virulence of bacterial pathogens. The first OMV vaccines successfully stopped Neisseria meningitidis serogroup B outbreaks but required detergent-extraction for endotoxin removal. Current vaccines use attenuated endotoxin, to preserve immunological properties and allow a detergent-free process. The...
متن کاملbiological and immunological evaluation of neisseria meningitidis serogroup a outer membrane vesicle as vaccine candidates
conclusions the results showed that the extracted omvs were conformationally stable, and there were no pyrogenic determinants in omv. also the results showed that the omv elicited high level of specific antibodies against n. meningitidis serogroup a. these results indicate that the omv obtained here, can be used as a meningococcal vaccine after further investigation. materials and methods omvs ...
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Neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in humans. Currently, there are no vaccines to prevent disease caused by strains of N.meningitidis serogroup B. The Class 1 Outer Membrane Protein (OMP) has been named porA which is a cation selective transmembrane protein of 45 KDa that forms trimeric pore in the meningococcal outer membrane. PorA from ser...
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An improved detergent-free process has been developed to produce vaccine based on native outer membrane vesicles (NOMV) against Neisseria meningitidis serogroup B. Performance was evaluated with the NonaMen vaccine concept, which provides broad coverage based on nine distinct PorA antigens. Scalable aseptic equipment was implemented, replacing undesirable steps like ultracentrifugation, inactiv...
متن کاملBiofilm formation by Neisseria meningitidis.
Biofilm formation by the human pathogen Neisseria meningitidis was analyzed. Biofilm-forming meningococcal strains were identified and quantitated by crystal violet staining. Laser scanning confocal microscopy of the meningococcal biofilm revealed variable layers up to 90 microm in thickness. A total of 39 meningococcal isolates were studied; 23 were nasopharyngeal-carriage isolates, and 16 wer...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2018
ISSN: 1475-2859
DOI: 10.1186/s12934-018-1007-7